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Description
O'GeneRuler 100 bp DNA Ladder, ready-to-use, is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose or polyacrylamide gels. The ladder is a mixture of chromatography-purified individual DNA fragments.
O'GeneRuler 100 bp DNA Ladder, ready-to-use, is premixed with 6X Orange DNA Loading Dye for direct loading on gel. 6X Orange DNA Loading Dye contains xylene cyanol FF and orange G. In a 1% agarose gel orange G dye migrates at 50 bp.
The ladder is supplied with 6X Orange DNA Loading Dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Features
Ideal for both DNA sizing and approximate quantification.
Sharp bands.
Bright reference bands (given in red).
Can be directly loaded and are stable at room temperature for 6 months.
Supplied with loading dye for sample DNA.
O'GeneRuler Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF and 10% glycerol.
6X Orange DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months. For longer periods store at -20°C. |