|
Description
FastRuler Low Range DNA Ladder is specifically designed for fast sizing and approximate quantification of double-stranded DNA in 48 well (or 96-well) high throughput gels, as well as in conventional agarose gels. The ladder is a mixture of five blunt-ended chromatography-purified individual DNA fragments that are easily resolved in a short separation distance (10-20 mm) after an 8-14 min run on the appropriate agarose gel.
The ladder is especially useful for electrophoresis of PCR products.
Features
Fast separation (8-14 min).
Short separation distance (10-20 mm).
Sharp bands, easy-to-remember fragment sizes and quantities.
Ready-to-use – premixed with loading dye.
Supplied with loading dye solution for sample DNA.
Quality Control
Well-defined bands are formed during 8-14 min agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.005% bromophenol blue, 10% glycerol.
6X MassRuler DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C. |