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Description
O'RangeRuler 50 bp DNA Ladder is designed for precise sizing of PCR products and other double-stranded DNA fragments in agarose or non-denaturing polyacrylamide gels. It is step ladder with 50 bp differences between the DNA fragments. It consists of purified and ligated blunt ended basic unit repeats of 50 bp. Not recommended for DNA quantification.
O'RangeRuler 50 bp DNA Ladder is supplied with 6X Orange DNA Loading Dye for sample DNA. Orange G dye can be used to monitor DNA migration in agarose or polyacrylamide gels and migrates at 50 bp in a 1% agarose gel. It is the preferable dye when visualization of small DNA fragments is important.
Features
Step ladder with 50 bp increments.
Sharp bands, easy-to-remember fragment sizes.
Evenly spaced reference bands (given in red).
Ready-to-use – premixed with 6X Orange Loading Dye for direct loading and room temperature storage.
Supplied with loading dye for sample DNA.
Quality Control
Well-defined bands are formed during agarose gel electrophoresis.
The absence of nucleases is confirmed by a direct nuclease activity assay.
Storage and Loading Buffer
10 mM Tris-HCl (pH 7.6), 10 mM EDTA, 0.025% orange G, 0.005% xylene cyanol FF, 10% glycerol.
6X Orange DNA Loading Dye
10 mM Tris-HCl (pH 7.6), 0.15% orange G, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA.
Storage
Store at room temperature or at 4°C for up to 6 months.
For longer periods, store at -20°C.
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。 |