说明: GeneRuler DNA Ladder Mix is recommended for sizing and approximate quantification of a wide range of double-stranded DNA on agarose gels. The ladder is a mixture of chromatography-purified individual DNA fragments. GeneRuler DNA Ladder Mix can be labeled radioactively with T4 Polynucleotide Kinase, see protocol. The ladder is supplied with 6X DNA Loading Dye. 特性: Ideal for both DNA sizing and approximate quantification. Sharp bands. Bright reference bands (given in red). Supplied with loading dye for sample DNA. 质量控制: Well-defined bands are formed during agarose gel electrophoresis. The DNA concentration is determined spectrophotometrically. The absence of nucleases is confirmed by a direct nuclease activity assay. Storage Buffer (TE buffer) 10 mM Tris-HCl (pH 7.6) and 1 mM EDTA. 6X DNA Loading Dye 10 mM Tris-HCl (pH 7.6), 0.03% bromophenol blue, 0.03% xylene cyanol FF, 60% glycerol and 60 mM EDTA. |