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说明：
dUTP (2'-deoxyuridine 5'-triphosphate) is supplied as 100 mM aqueous solution titrated to pH 7.0 with NaOH.
General Characteristics
lambdamax = 262 nm,
extinction coefficient = 10.0x103 M-1 x cm-1 (pH 7.0).
特性：
Greater than 99% purity confirmed by HPLC.
Free of human and E.coli DNA.
Highly stable – the neutral pH of nucleotide solutions ensures the stability during long-term storage.
Application tested in:
– long range PCR (40 kb),
– cDNA synthesis and RT-PCR,
– real-time PCR,
– standard PCR,
– high fidelity PCR.
应用：
For use in all molecular biology applications including PCR, real-time PCR and RT-PCR with Taq DNA Polymerase, cDNA synthesis and primer extension.
质量控制：
Functionally tested in PCR.
Greater than 99% purity confirmed by HPLC.
Free of endo-, exodeoxyribonuclease, ribonuclease and nicking activities.
注意：
dUTP is not suitable for PCR with proofreading enzymes or enzyme mixes containing proofreading enzymes. |