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TatI现货进口
Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 65°C. To ensure higher efficiency of digestion, perform the cleavage reaction under paraffin oil.
Storage Buffer
TatI is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 2-fold overdigestion with TatI, more than 95% of the DNA fragments can be ligated and recut. ER1291 100 u (5 u/µl) 1.00 ml ER1291
ER1292 500 u (5 u/µl) ER1292 |