现货|SduI (Bsp1286I)|SduI Bsp1286I 产地:MBI 货号:ER0651 Storage Buffer SduI is supplied in: 10 mM Tris-HCl (pH 7.4 at 25°C), 100 mM KCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% (v/v) glycerol. Ligation and Recleavage After 50-fold overdigestion with SduI, more than 95% of the DNA fragments can be ligated and recut. Conditions for 100% Activity 1X Buffer SduI: 10 mM Tris-HCl (pH 7.2 at 37°C), 3 mM MgCl2, 150 mM NaCl and 0.1 mg/ml BSA. Incubate at 37°C. |