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SalI 进口原装
产地：MBI
货号：ER0641
Storage Buffer
SalI is supplied in:
10 mM Tris-HCl (pH 7.4 at 25°C),100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with SalI, more than 95% of the DNA fragments can be ligated and recut.
Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C. |