Hin6I (Hin1PI) 产地:MBI 货号:ER0481 Conditions for 100% Activity 1X Buffer Tango: 33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA. Incubate at 37°C. Storage Buffer Hin6I is supplied in: 10 mM potassium phosphate (pH 7.4 at 25°C), 100 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/ml BSA and 50% (v/v) glycerol. Ligation and Recleavage After 50-fold overdigestion with Hin6I, more than 95% of the DNA fragments can be ligated and recut. Isoschizomers Search for commercial isoschizomers using REsearch™. |