Eco130I (StyI) 产地:MBI 货号:ER0411 Conditions for 100% Activity 1X Buffer O: 50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA. Incubate at 37°C. Storage Buffer Eco130I is supplied in: 10 mM Tris-HCl (pH 7.5 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol. Ligation and Recleavage After 50-fold overdigestion with Eco130I, more than 95% of the DNA fragments can be ligated and recut. Digestion of Agarose-embedded DNA Minimum 5 units of the enzyme are required for complete digestion of 1 µg of agarose-embedded lambda DNA in 16 hours. |