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Eco31I (BsaI)
产地：MBI
货号：ER0291
Conditions for 100% Activity
1X Buffer G:
10 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 50 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Eco31I is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 200 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Eco31I, more than 95% of DNA fragments can be ligated and recut. |