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Cfr9I (XmaI)
产地：MBI
货号：ER0171
Conditions for 100% Activity
1X Buffer Cfr9I:
10 mM Tris-HCl (pH 7.2 at 37°C), 5 mM MgCl2, 200 mM sodium glutamate and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Cfr9I is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 250 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Cfr9I, more than 95% of the DNA fragments can be ligated and recut. |