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Bsu15I (ClaI)
产地：MBI
货号：ER0142
Conditions for 100% Activity
1X Buffer Tango™:
33 mM Tris-acetate (pH 7.9 at 37°C), 10 mM Mg-acetate, 66 mM K-acetate and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Bsu15I is supplied in:
10 mM Tris-HCl (pH 8.0 at 25°C), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5 mg/ml BSA and 50% (v/v) glycerol.
Ligation and Recleavage
After 50-fold overdigestion with Bsu15I, more than 95% of the DNA fragments can be ligated and recut. |