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Alw21I (BsiHKAI)
产地：MBI
货号：ER0021
Product information
Reaction conditions
Recommended buffer for 100% activity Optimal tempThermo Scientificure Enzyme activity in Thermo Scieitnfic buffers, % Tango™ buffer for double digestion
B (blue)
1X G (green)
1X O (orange)
1X R (red)
1X Tango™ (yellow)
1X / 2X
O 37°C 0-20 20-50 100 50-100 20-50 50-100 1X or 2X
Lambda DNA
1.0% agarose
28 cleavage sites
Conditions for 100% Activity
1X Buffer O:
50 mM Tris-HCl (pH 7.5 at 37°C), 10 mM MgCl2, 100 mM NaCl and 0.1 mg/ml BSA.
Incubate at 37°C.
Storage Buffer
Alw21I is supplied in:
10 mM Tris-HCl (pH 7.5 at 25°C), 300 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.2 mg/ml BSA and 50% (v/v) glycerol. |