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DreamTaq Green DNA Polymerase
产地：MBI
货号：EP0712
Applications
Routine PCR amplification of DNA fragments up to 6 kb.
RT-PCR.
Genotyping.
Generation of PCR products for TA cloning.
Description
DreamTaq™ Green DNA Polymerase is a combination of DreamTaq™ DNA Polymerase and 10X DreamTaq™ Green Buffer. DreamTaq™ DNA Polymerase is an enhanced Taq polymerase optimized for high throughput PCR applications. It ensures higher sensitivity, longer PCR products and higher yields compared to conventional Taq DNA polymerase. DreamTaq™ generates PCR products with 3'-dA overhangs and does not incorporate dUTP.
The 10X DreamTaq™ Green Buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with PCR performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless 10X DreamTaq™ Buffer (#B65) or purifying the PCR product prior to analysis. The 10X DreamTaq™ Green Buffer is optimized for robust performance in PCR and includes MgCl2 at a concentration of 20 mM. |