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Description
TrueStart™ Hot Start Taq DNA Polymerase is designed for hot start PCR, a technique that has been shown to improve specificity, sensitivity and yield of DNA amplification during PCR (1-5). TrueStart™ Hot Start Taq DNA Polymerase has been chemically modified by the addition of proprietary heat-labile blocking groups to amino acid residues. Because of this modification, TrueStart™ Hot Start Taq DNA Polymerase is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers. TrueStart™ Hot Start Taq DNA Polymerase is rapidly activated during the initial denaturation step of PCR. The lack of additional activation step differentiates it from other hot start DNA polymerases and makes the enzyme an ideal tool for both automatic hot start PCR and fast PCR. Activated TrueStart™ Hot Start Taq DNA Polymerase catalyzes 5'=>3' synthesis of DNA, has no detectable 3'=>5' exonuclease (proofreading) activity, but possesses low 5'=>3' exonuclease activity. Before activation the two activities are not detectable.
Applications
High throughput hot start PCR.
RT-PCR.
Highly specific amplification of genomic and cDNA targets up to 3 kb.
Amplification of low copy DNA targets.
Real-time PCR.
Multiplex PCR.
Generation of PCR product for TA cloning.
Storage Buffer
The enzyme is supplied in:
20 mM Tris-HCl (pH 8.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween 20, 0.5% (v/v) Nonidet P40 and 50% (v/v) glycerol. |