正品 Pfu DNA Polymerase (recombinant)

Description
Pfu DNA Polymerase is a highly thermostable DNA polymerase from the hyperthermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'=>3' direction. Pfu DNA Polymerase also exhibits 3'=>5' exonuclease (proofreading) activity, that enables the polymerase to correct nucleotide incorporation errors. It has no 5'=>3' exonuclease activity.
Applications
High fidelity PCR.
Generation of PCR products for cloning and expression.
RT-PCR for cDNA cloning and expression.
Blunt-end PCR cloning (1).
Site-directed mutagenesis.
Quality Control
The absence of endo- and exodeoxyribonucleases confirmed by appropriate quality tests. Functionally tested in PCR.