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Terminal Deoxynucleotidyl Transferase
产地：MBI
货号：EP0161
Applications
Production of synthetic homo- and heteropolymers (1).
Homopolymeric tailing of linear duplex DNA with any type of 3'-OH terminus (2, 3).
Oligodeoxyribonucleotide and DNA labeling (2, 4-8).
5'-RACE (Rapid Amplification of cDNA Ends) (9).
In situ localization of apoptosis (10).
Description
Terminal Deoxynucleotidyl Transferase (TdT), a template-independent DNA polymerase, catalyzes the repetitive addition of deoxyribonucleotides to the 3'-OH of oligodeoxyribonucleotides and single-stranded and double-stranded DNA (1). TdT requires an oligonucleotide of at least three nucleotides to serve as a primer. With RNA as template TdT shows variable performance which strongly depends upon the tertiary structure of acceptor RNA 3'-end and the nature of nucleotide. Generally, it is lower than using DNA as a template. |