Klenow Fragment 产地:MBI 货号:EP0052ApplicationsDNA blunting by fill-in 5'-overhangs (1).Random-primed DNA labeling (2-4).Labeling by fill-in 5'-overhangs of dsDNA.DNA sequencing by the Sanger method (5).Site-specific mutagenesis of DNA with synthetic oligonucleotides (6).Second strand synthesis of cDNA (7).DescriptionKlenow Fragment is the Large Fragment of DNA Polymerase I. It exhibits 5'=>3' polymerase activity and 3'=>5' exonuclease (proofreading) activity, but lacks 5'=>3' exonuclease activity of DNA polymerase I.