正品|Exonuclease I, E.coli

Exonuclease I
产地：MBI
货号：EN0582
Applications
Primer removal from PCR mixtures:
– prior to PCR product sequencing (2),
– for one-tube "megaprimer" PCR mutagenesis (3).
Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.
Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4).
Description
Exonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact, see Fig.1.
It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).
注：本产品仅用于生命科学研究实验，不作为任何药物及其他用途。