Exonuclease I产地:MBI 货号:EN0582ApplicationsPrimer removal from PCR mixtures:– prior to PCR product sequencing (2),– for one-tube "megaprimer" PCR mutagenesis (3).Removal of single-stranded DNA containing a 3'-hydroxyl terminus from nucleic acid mixtures.Assay for the presence of single-stranded DNA with a 3'-hydroxyl terminus (4).DescriptionExonuclease I (ExoI) degrades single-stranded DNA in a 3'=>5' direction, releasing deoxyribonucleoside 5'-monophosphates in a stepwise manner and leaving 5'-terminal dinucleotides intact, see Fig.1.It does not cleave DNA strands with terminal 3'-OH groups blocked by phosphoryl or acetyl groups (1).注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。