RNase A, DNase and protease-free 产地:MBI 货号:EN0531 Applications Plasmid and genomic DNA preparation (3, 4). Removal of RNA from recombinant protein preparations. Ribonuclease protection assays. Used in conjunction with RNase T1 (3). Mapping single-base mutations in DNA or RNA (5, 6). Description RNase A, DNase and protease-free is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues, see Fig.1. It cleaves the phosphodiester bond between the 5'-ribose of a nucleotide and the phosphate group attached to the 3'-ribose of an adjacent pyrimidine nucleotide. The resulting 2', 3'-cyclic phosphate is hydrolyzed to the corresponding 3'-nucleoside phosphate (1, 2). |