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DNase I, RNase-free|正品进口

产品编号: EN0521     查看说明书
产品名称: DNase I, RNase-free|正品进口  .0   订购此产品 
供应商: MBI
规格: 1000 units
目录价: 417.8475
库存状态: 现货
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DNase I, RNase-free
产地:MBI
货号:EN0521
Applications
Preparation of DNA-free RNA (1).
Removal of template DNA following in vitro transcription (1).
Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR (2).
DNA labeling by nick-translation in conjunction with DNA Polymerase I (1).
Studies of DNA-protein interactions by DNase I, RNase-free footprinting (1).
Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used (3).
Description
DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups.
The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions, see Fig.1:
– in the presence of Mg2+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion (1);
– in the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides (1).
本产品仅用于生命科学研究实验,不作为任何药物及其他用途。

保存条件: RT
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