|
|
 |
您现在的位置:产品中心 >>
分子生化试剂 >> 工具酶 |
DNase I, RNase-free|正品进口
产品编号: |
EN0521
查看说明书 |
产品名称:
|
DNase I, RNase-free|正品进口 .0 订购此产品 |
供应商:
|
MBI |
规格: |
1000 units |
目录价: |
417.8475 |
库存状态: |
现货 |
CAS编号:
|
|
应用范围: |
|
种属来源: |
|
相关信息: |
|
DNase I, RNase-free 产地:MBI 货号:EN0521 Applications Preparation of DNA-free RNA (1). Removal of template DNA following in vitro transcription (1). Preparation of DNA-free RNA prior to RT-PCR and RT-qPCR (2). DNA labeling by nick-translation in conjunction with DNA Polymerase I (1). Studies of DNA-protein interactions by DNase I, RNase-free footprinting (1). Generation of a library of randomly overlapping DNA inserts. Reaction buffer containing Mn2+ is used (3). Description DNase I, RNase-free is an endonuclease that digests single- and double-stranded DNA. It hydrolyzes phosphodiester bonds producing mono- and oligodeoxyribonucleotides with 5'-phosphate and 3'-OH groups. The enzyme activity is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions, see Fig.1: – in the presence of Mg2+, DNase I cleaves each strand of dsDNA independently, in a statistically random fashion (1); – in the presence of Mn2+, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides (1). 本产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |
保存条件: |
RT |
说明书地址: |
点击查看详细 |
|
|
打印此页 关闭此页 |
上一个:Uracil-DNA Glycosylase[EN0362/5x200 units]
下一个:DNase I, RNase-free, HC[EN0523/1000 units]
|
|
 |
 |
|