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S1 Nuclease
产地：MBI
货号：EN0321
Applications
Removal of single-stranded overhangs of DNA fragments (2).
S1 transcript mapping (3, 4).
Cleavage of hairpin loops.
Creation of unidirectional deletions in DNA fragments in conjunction with Exonuclease III (5).
Description
S1 Nuclease degrades single-stranded nucleic acids, releasing 5'-phosphoryl mono- or oligonucleotides, see Fig.1. It is five times more active on DNA than on RNA (1). S1 Nuclease also cleaves dsDNA at the single-stranded region caused by a nick, gap, mismatch or loop. S1 Nuclease exhibits 3'-phosphomonoesterase activity.
The enzyme is a glycoprotein with carbohydrate content of 18%. |