Ribonuclease H 产地:MBI 货号:EN0201ApplicationsRemoval of mRNA prior to synthesis of second strand cDNA (1).RT-PCR and qRT-PCR: removal of RNA after first strand cDNA synthesis.Removal of the poly(A) sequences of mRNA after hybridization with oligo(dT) (2).Site-specific cleavage of RNA (3).Studies of in vitro polyadenylation reaction products (4).DescriptionRibonuclease H (RNase H) specifically degrades the RNA strand in RNA-DNA hybrids, see Fig.1. It does not hydrolyze the phosphodiester bonds within single-stranded and double-stranded DNA and RNA.