T4 DNA Ligase 产地:MBI 货号:EL0012 Applications Cloning of restriction enzyme generated DNA fragments. Cloning of PCR products. Joining of double-stranded oligonucleotide linkers or adaptors to DNA. Site-directed mutagenesis. Amplified fragment length polymorphism (AFLP). Ligase-mediated RNA detection (3). Nick repair in duplex DNA, RNA or DNA/RNA hybrids. Self-circularization of linear DNA. Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids (1, 2). The T4 DNA Ligase requires ATP as cofactor. |