10X T4 DNA Ligase Buffer 产地:MBI 货号:B69 Quality Control The absence of endo-, exodeoxyribonucleases, phosphatases and ribonucleases confirmed by appropriate quality tests. Functionally tested for the capacity to join cohesive- and blunt-end DNA fragments. Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA. The enzyme repairs single-strand nicks in duplex DNA, RNA or DNA/RNA hybrids, joins DNA fragments with either cohesive or blunt termini, but has no activity on single-stranded nucleic acids (1, 2). The T4 DNA Ligase requires ATP as cofactor. 注:本产品仅用于生命科学研究实验,不作为任何药物及其他用途。 |