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50X TAE Buffer (Tris-acetate-EDTA)
产地：MBI
货号：B49
Product information
Applications
Electrophoresis of nucleic acids in agarose and polyacrylamide gels.
Used both as a running buffer and as a gel preparation buffer.
Filtered through a 0.22 µm membrane.
Recommended for resolution of RNA and DNA fragments larger than 1500 b(p), for genomic DNA and for large supercoiled DNA.
1X Composition
40 mM Tris,
20 mM acetic acid,
1 mM EDTA,
pH of 50X TAE: 8.4
Usage Recommendations
Use fresh 1X TAE both for the gel and for the electrophoresis run.
To prepare 1X TAE buffer add 20 ml of 50X TAE buffer to 980 ml of deionized water and mix well.
Quality Control
The absence of endo-, exodeoxyribonucleases and ribonucleases confirmed by appropriate quality tests.
Storage
There are no time limitations for storage of the electrophoresis buffers at room temperature. If the buffer is stored at lower temperatures, a precipitate may form, which is easily dissolved by gentle heating.
本产品仅用于生命科学研究实验。 |