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Tissues can contain endogenous alkaline phosphatase activity that will produce background staining if an alkaline phosphatase detection system and substrate are used. To eliminate this problem, Levamisole Solution, an inhibitor of alkaline phosphatase, is provided in a convenient dropper bottle and can be added to the alkaline phosphatase substrate solution. Levamisole does not inhibit the isoenzyme used for either the VECTASTAIN® ABC-AP reagents or other alkaline phosphatase conjugates. 100x Concentrate.
This reagent is supplied as a 100X concentrate. Provided in a convenient drop dispensing bottle, one drop of levamisole concentrate is added to 5 ml of substrate solution. |