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Biotin (Long Arm) Maleimide is designed for biotinylating proteins, nucleic acids or other molecules containing one or more thiol groups. The presence of the 6-aminohexanoate spacer arm between the maleimide group and biotin reduces the possibility of steric hinderance. Molecules to be labeled with Biotin (Long Arm) Maleimide require free thiol groups. In the case of proteins, Biotin (Long Arm) Maleimide will react with exposed cysteine residues. Alternatively, free thiols may be generated by reducing disulfide bonds or by modifying other reactive groups such as priamry amines with componds like Traut’s reagent (2-iminothiolane).
For labeling nucleic acids, thiols can be introduced into DNA, RNA, or oligonucleotides using the FastTag®, 5’EndTag™ or 3’ EndTag™ nucleic acid labeling systems. |