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Used as anterograde tracers, our Biotinylated Dextran Amines (BDA) are of approximately 10,000 MW and are conjugated with either fluorescein or Texas Red®. These tracers can be effectively introduced by iontophoretic or pressure injection methods. The injection site can then be examined easily with the fluorescent microscope while details of labeled fibers and terminals can be observed after VECTASTAIN® ABC - Peroxidase and substrate (DAB, Vector® SG or Vector® VIP) development. In addition, these BDAs can be used in double labeling protocols in conjunction with anterograde or retrograde HRP labeling or combined with immunohistochemistry for other substances. |