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Vector® VIP and ImmPACT™ VIP substrates both produce intense, violet reaction products, and can be used as alternatives to DAB or as a second color for multiple antigen labeling. Vector® VIP and ImmPACT™ VIP substrates also provide excellent color contrast in pigmented tissues such as melanoma. With the aid of imaging systems and software, the spectral profile of both substrates can be distinguished from other enzyme substrates in applications where antigens are co-localized. Sections stained with either substrate can be viewed by darkfield and electron microscopy. Vector® VIP and ImmPACT™ VIP substrates can be used for both manual and automated staining methods. Stained sections should be dehydrated, cleared, and permanently mounted.
Vector® VIP Substrate Kit contains stock solutions in convenient dropper bottles. The sensitivity of Vector® VIP substrate is equivalent to Vector® DAB (SK-4100). This product can also be used on blots. The kit provides all of the necessary reagents to prepare about 300 ml of working solution. When stored at 4 ºC, this kit is stable for one year. |