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GSL I is a family of glycoproteins with molecular weights of approximately 115,000. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for a-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for a-galactose residues. Our GSL I is a mixture of the five isolectins. Binding appears to require divalent cations such as calcium and magnesium. GSL I has been reported to bind several glycoproteins including laminin.
Rhodamine labeled Griffonia Simplicifolia Lectin I is produced by using the highest quality tetramethylrhodamine isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Rhodamine labeled Griffonia Simplicifolia Lectin I has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Rhodamine labeled Griffonia Simplicifolia Lectin I has an absorption maximum at about 550 nm and a maximum emission at about 575 nm. |