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Ulex europaeus agglutinin I is a glycoprotein with a molecular weight of 63,000, although multimeric aggregates have been reported. UEA I has two subunits, one of about 31,000 daltons and another of 32,000 daltons. UEA I binds to many glycoproteins and glycolipids containing a -linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.
,br> Rhodamine labeled Ulex Europaeus Agglutinin I is produced by using the highest quality tetramethylrhodamine isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Rhodamine labeled Ulex Europaeus Agglutinin I has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Rhodamine labeled Ulex Europaeus Agglutinin I has an absorption maximum at about 550 nm and a maximum emission at about 575 nm.
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions perserved with sodium azide. |