Our high affinity Horse Anti-Goat IgG Antibody is purified by affinity chromatography and adsorbed to remove any cross-reactivities that are likely to interfere with specific labeling. This antibody is then cross-linked with the highest specific activity horseradish peroxidase. A proprietary procedure for conjugation allows the maximum preservation of enzyme activity and antibody specificity. This antibody is subjected to rigorous quality control assays and can be used for tissue staining, ELISAs, and blots. As well as recognising goat IgG, this antibody is suitable to detect bovine IgG primary antibodies. The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker solution (Cat. No. SP-5030) is recommended.