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Sequence specific DNA ligands bind to GC- or AT-rich sites on DNA and retard the electrophoretic migration of DNA in a sequence specific manner. This ability is critical for applications such as differential display in which multiple DNA species of similar size need to be separated before subsequent excision and analysis. The Resolve-It™ Kit contains two ligands. AT-Yellow™, a bisbenzimide-PEG conjugate, similar to the discontinued HA-Yellow, binds to AT-rich regions; GC-Red™, a phenyl neutral red-PEG conjugate, similar to HA-Red binds GC-rich regions. Either ligand is added to the agarose during gel preparation, and, during electrophoresis, ligand interaction retards the mobility of DNA depending on the amount of ligand bound. The quantity of each ligand supplied is sufficient to prepare about 200 ml of agarose gel. |