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VECTASHIELD Mounting Medium with DAPI /防荧光萃灭；含DAPI,可衬染细胞核
VECTASHIELD® Mounting Media are unsurpassed in preventing photobleaching. The different formulations of VECTASHIELD® Mounting Media all offer the same outstanding anti-fade and anti-photobleaching properties. They are all compatible with fluorescein, Texas Red®, AMCA, DyLight® dyes, Alexa Fluor® dyes, fluorescent nuclear stains, fluorescent proteins, fluorescent tracers, histochemical stains, and most fluorochromes.
Features of VECTASHIELD® Mounting Media:
Inhibit photobleaching of most fluorochromes
Available in non-hardening or hardening formulations
Available with or without DAPI or propidium iodide
No warming necessary
Slides can be viewed after prolonged storage
Continues to inhibit photobleaching after prolonged storage of mounted slides
Easy to use
Optically clear
Ideal refractive index
The original VECTASHIELD® Mounting Medium is a glycerol-based, aqueous mountant that does not solidify but remains a viscous liquid on the slide. After mounting, coverslipped slides will not readily dry out and can be reviewed for weeks afterwards without sealing. For prolonged storage, coverslips can be permanently sealed around the perimeter with nail polish. Mounted slides should be stored at 4 ºC.
Both the VECTASHIELD® HardSet™ and the original VECTASHIELD® Mounting Media are available with or without the counterstain DAPI (4’, 6-diamidino-2-phenylindole). DAPI produces a blue fluorescence when bound to DNA with excitation at about 360 nm and emission at 460 nm.
VECTASHIELD® Mounting Media are compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins. Please consult the compatibility table (located in product information box) to determine if VECTASHIELD® will be compatible in your system.
Please note: VECTASHIELD® Mounting Medium may not be compatible with all enzymatic substrates or fluorescent proteins and test sections would be advisable when using anything other than commonly used fluorochromes.
The refractive index for VECTASHIELD® Mounting Medium is 1.44.
1R.J. Florijn, et. al. Cytometry, 19 (1995) 177-182. |