Succinylated Con A is made from our highly purified Con A and has many unusual properties, such as failure to induce patch and cap formation and at high concentrations does not show a diminished mitogenic effect. In addition, succinylated Con A affects cyclic nucleotide levels in lymphocytes differently than does native Con A. Fluorescein labeled, succinylated Concanavalin A is produced by using the highest quality fluorescein isothiocyanate, our affinity-purified lectin, and special conjugation procedures. Fluorescein labeled succinylated Concanavalin A has an appropriate number of fluorochromes bound which provide the maximum fluorescence and optimum staining characteristics for this particular lectin. This lectin is supplied essentially free of unconjugated fluorochromes and inactive lectin. Fluorescein labeled succinylated Concanavalin A has an excitation at 495 nm and an emission at about 515 nm. |