Fluorescein Rabbit Anti-Goat IgG Antibody can be used for flow cytometry or for tissue staining. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining. Maximum excitation is at 495 nm and maximum emission is at 515 nm. As well as recognising goat IgG, this antibody is suitable to detect bovine IgG primary antibodies. The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker solution (Cat. No. SP-5030) is recommended.