AMCA (7-amino-4-methylcoumarin-3-acetic acid) labeled Rabbit Anti-Goat IgG Antibody excites in the near ultraviolet (350 nm) and emits a visible bright blue fluorescence at about 450 nm. This antibody has been optimally labeled with AMCA to provide the brightest label for fluorescence microscopy. As well as recognising goat IgG, this antibody is suitable to detect bovine IgG primary antibodies. The use of bovine products such as casein, serum, albumin or non-fat dry milk as blocking agents in ELISA or blot assays may produce a high background due to cross-reactivity of this antibody with bovine immunoglobulins that may be present. If this antibody is to be used in these applications, blocking with Animal-Free Blocker solution (Cat. No. SP-5030) is recommended.