Biotinylated Horse Anti-Mouse IgG, rat adsorbed, is supplied in lyophilized form and can be reconstituted with 1 ml water. With some exceptions the recommended dilution for most applications is 1:100.(H+L) indicates the antibody recognizes both heavy and light chains. This specially prepared antibody is designed to be used on tissues that may contain interfering endogenous immunoglobulins. The rat adsorbed anti-mouse IgG (H+L) products are designed for use with rat tissues that may contain endogenous rat immunoglobulins. This adsorbed antibody allows primary antibodies made in mouse to be used on rat tissues.