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Antibodies to lectins are produced by hyperimmunizing goats with our highly purified lectins. Specific antibodies are isolated by affinity chromatography on lectin-agarose columns, and then optimally labeled with biotin. Biotinylated anti-lectins can be used as intermediates to localize lectin receptors in tissues or on cells, or to follow lectin migration in neuronal transport. In some applications, biotinylated anti-lectins used in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone. |