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This lectin has a molecular weight of 40,000-46,000 and is composed of four subunits. There appear to be two different chains in combinations giving rise to five isolectins. The isoelectric point of this lectin is pH 4.7, and it is probably devoid of carbohydrate or metal ions. The carbohydrate specificity of either type of subunit is likely to be the same, preferring alpha linked N-acetylgalactosamine structures.
This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. |