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PHA-L, although a potent mitogen, has a rather narrow range of concentrations for mitogenic stimulation and appears to require calcium and manganese ions for activity. The presence of some “E” subunits in PHA appears to broaden the concentration range of maximum mitogenic stimulation. As a consequence, PHA (E+L) may be preferred for general mitogenic assays. PHA-L has been found to be an excellent, specific marker for use in anterograde neuronal tracing.
This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. |