Succinylated Con A is made from our highly purified Con A and has many unusual properties, such as failure to induce patch and cap formation and at high concentrations does not show a diminished mitogenic effect. In addition, succinylated Con A affects cyclic nucleotide levels in lymphocytes differently than does native Con A. This biotinylated lectin conjugate is prepared from affinity-purified lectin and is optimally labeled with biotin. Essentially free of inactive lectin conjugate and containing no free biotin, this biotinylated lectin provides an ideal intermediate for examining glycoconjugates using the Biotin-Avidin System. First the biotin-labeled lectin is added, followed by the VECTASTAIN® ABC Reagent, Avidin D conjugate, or streptavidin derivative. Another possible application is in the isolation of lymphokines and other products of mitogenic stimulation. |