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Succinylated Con A is made from our highly purified Con A and has many unusual properties, such as failure to induce patch and cap formation and at high concentrations does not show a diminished mitogenic effect. In addition, succinylated Con A affects cyclic nucleotide levels in lymphocytes differently than does native Con A.
Agarose bound* Succinylated Concanavalin A is prepared from affinity-purified lectin. Heat stable, cross-linked 4% agarose beads with a molecular weight exclusion limit of about 2x107 are used as the solid-phase matrix to which the lectin is covalently bound. The attachment of the lectin to the solid phase is carefully controlled in order to preserve the activity of the lectin as well as to minimize conformational changes of the bound lectin which might result in nonspecific ionic or hydrophobic interactions. |