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Greater sensitivity can be achieved by using an amplified detection procedure. Our biotin/avidin and biotin/streptavidin systems introduce a large number of enzymes to the target site providing signal amplification while maintaining low background.
The VECTASTAIN® ABC-AmP™ is an amplified ABC alkaline phosphatase reagent for the detection of mouse or rabbit primary antibodies on nitrocellulose or PVDF membranes. The signal can be visualized using either a chemiluminescent/ chemifluorescent or a chromogenic substrate. Used with the DuoLuX™ Chemiluminescent/Chemifluorescent Substrate, the VECTASTAIN® ABC- AmP™ system produces a very high and sustained light emission signal, with low background, and permanent fluorescence.
The sensitivity of VECTASTAIN® ABC-AmP™ Reagent combined with the DuoLux™ substrate allows for the detection of as little as 1 pg of target protein. |