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Vector Laboratories’ enzyme-conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios. Specific covalent linkages are chosen to provide stable, highly active conjugates.
These enzyme conjugates are suitable for use in solid-phase assays, tissue/cell staining systems, and blotting applications.
The β-galactosidase used to prepare β-galactosidase Avidin D is isolated from a special strain of E. coli. The enzyme has an exceptionally high specific activity, allowing a greater degree of sensitivity in assays. Our β-galactosidase has an alkaline pH optimum, unlike the β-galactosidases present in mammalian tissues which all have acidic optima. This reagent can be employed in many solid-phase assays or immunohistochemical systems without interference from endogenous enzyme activity present in the tissues.
One unit is 1 µmole o-nitrophenyl-β-D-galactopyranoside hydrolyzed per min. at 37 °C, pH 7.3. |