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VECTREX® Avidin D (A-2020) is used for irreversible binding of biotinylated nucleic acids. This product consists of Avidin D covalently linked to the surface of the VECTREX® matrix.
VECTREX® Avidin D is useful in applications such as subtractive hybridization. A probe that is biotinylated with PHOTOPROBE®, FastTag®, or 3’ or 5’ EndTag™ Labeling Systems is hybridized to a mixture of nucleic acid in solution. After hybridization, the mixture is incubated with a suspension of VECTREX® Avidin D or passed through a column containing VECTREX® Avidin D. The DNA or RNA that is not associated with the biotinylated probe is left in solution or passes through the column. The biotinylated probe and the nucleic acid complementary to the probe are thus separated from the noncomplementary sequences. This matrix is supplied as a 1:1 suspension in buffer. The binding capacity is at least 22 ng of biotin-labeled λ DNA per μl of 1:1 suspension. |