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Pyruvate Colorimetric/Fluorometric Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Application- The kit detects 1 µM to 10 mM pyruvate.
Features & Benefits:
• Simple procedure; takes ~40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of pyruvate concentrations in biological samples
Kit components:
• Pyruvate Assay Buffer
• Pyruvate Probe (in DMSO)
• Pyruvate Enzyme Mix
• Pyruvate Standard (100 nmol/µl)
Description:
Pyruvate is a central molecule in metabolism through which sugars enter the citric acid cycle. Pyruvate can be converted to carbohydrates during gluconeogenesis or to fatty acids via acetyl CoA. High levels of pyruvate are associated with liver disease and genetic disorders. Pyruvate has also been used to stimulate metabolism leading to loss of body weight. BioVision provides a simple, direct and automation-ready procedure for measuring pyruvate concentration in various biological samples such as blood, cells, culture and fermentation media, etc. In the assay, pyruvate is oxidized by pyruvate oxidase via enzyme reactions to generate color (λ= 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the color or fluorescence intensity is proportional to pyruvate content, the pyruvate concentration can be accurately measured. The kit detects 1 µM to 10 mM pyruvate.
Storage Conditions:
-20°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |