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Adiponectin (mouse) Elisa Assay Kit
Product Overview
Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- This mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants
Features & Benefits:
• Simple procedure
• Fast and convenient
Kit components:
• Antibody coated 96-well plate
• 10X Wash concentrate, 50 ml
• 5X Diluent, 50 ml
• Secondary antibody, 12 ml
• 100X Detector, 150 µl
• Standard, recombinant mouse adiponectin (16 ng), 1 vial,lyophilized
• QC sample= positive control having 1-1.5 ng of mouse serum adiponectin
• Substrate I, 6 ml
• Substrate II, 6 ml
• Stop solution, 12 ml
Description:
Adipose tissue secretes a number of biologically active soluble factors (collectively named adipocytokines) that regulate glucose and fatty acid metabolism. Measurement of serum adiponectin levels gives us important information on the role of adiponectin in regulation of glucose and/or lipid metabolism. This Mouse Adiponectin ELISA Kit is an enzyme-linked immunosorbent assay (ELISA) for quantitative determination of adiponectin in mouse serum, plasma or various tissue or cell culture supernatants. In the assay, monoclonal antibody specific for mouse adiponectin has been pre-coated onto 96 well microplate. Standards and samples are pipetted into the wells and adiponectin present is bound by immobilized antibody. The bound adiponectin is then captured by anti-mouse adiponectin polyclonal antibody. With HRP conjugated anti-rabbit IgG and a HRP substrate, the colors developed in proportion to the bound adiponectin, can be easily measured by Elisa plate reader.
Storage Conditions:
+4°C
Shipping Conditions:
gel pack
USAGE: For Research Use Only! Not For Use in Humans. |